IL21

The endogenous mouse Il21 gene was replaced by human IL21 gene.

Validation Data

Figure 1. Detection of IL21 expression in splenocyte  by RT-PCR. 

Wild type: only one band at 272 bp with primers F1/R1(mIl21); Homozygous: only one band at 304 bp with primers F2/R2(hIL21); Abbr. M, DNA marker; HO, homozygous; HE, heterozygous; WT, wild type.

Figure 2. Detection of hIL21 expression in splenocyte culture supernatants by ELISA (n=3). 

Abbr. HO, homozygous; HE, heterozygous; WT, wild type. Note. hIL21 and C57BL/6 mice were stimulated with ConA in vitro.