A CreERT2-Wpre-polyA expression cassette was knocked into initiation codon ATG site of the Krt14 gene via CRISPR/Cas mediated recombination.
Validation Data
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Figure 1: CreERT2-mediated recombination in Skin, Tongue & Esophagus of Krt14 CreERT2+/-; R26 tdTomato+/-Â mice.
A. Krt14-CreERT2+/-Â ; Rosa26 tdTomato+/-Â ; Tamoxifen
B. Krt14-CreERT2+/-; Rosa26 tdTomato+/-Â
C. Rosa26 tdTomato+/-
Figure 2: Confocal images showing expression of tdTomato in Skin, Tongue & Esophagus of Krt14 CreERT2+/-; R26 tdTomato +/- mice.
A. Krt14-CreERT2+/-Â ; Rosa26 tdTomato+/-Â ; Tamoxifen
B. Krt14-CreERT2+/-; Rosa26 tdTomato+/-Â
C. Rosa26 tdTomato+/-
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