TARGATT™ Site-specific Knock-in iPSC Service

Description

TARGATT™ Knock-in iPS Cell Service Details 

Integration of your gene of interest into our TARGATT™ Master Cell Line which contains a pre-engineered “attP” landing pad.

• Standard (TARGATT™ Master Human iPSC Line)
• Fully Customizable Service – Inquire

Standard Deliverables:

• Two (2) iPSC clones with two (2) vials per clone at 1 x 10^6 cells/ vial
• Clones are characterized for three (3) pluripotency markers: OCT4, SOX2, SSEA4, TRA-1-60, TRA-1-81
• Karyotyping Data 
  • Additional characterization services are available.
• Final Report

Standard Workflow and Timeline:

• Vector design, construction, and validation
• Transfection and optimization
• Screening for single-cell clones and clone confirmation
• Cell expansion and cryopreservation

Timeline: 3-5 months

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TARGATT™ Master Human iPSC Line

The TARGATT™ master iPSC line contains a “docking attP” site at the safe harbor genomic H11 locus. Any gene of interest (GOI) on an “attB” vector can be inserted efficiently at the H11 locus through phiC31 integrase mediated recombination between “attP” and “attB.” The efficiency of transgene insertion is up to 100% with drug selection and up to 30% without drug selection.

Application: 

• iPSC reporter lines for cell tracking
• iPSC lines with surface markers for cell purification
• iPSC lines with cell-specific promoters to direct iPSC differentiation

Projects:

• iPSC lines with excisable Cas9 or nickase for efficient CRISPR gene modification
• High-efficiency, high purity cells differentiated from iPSC lines

iPSC lines with cell-specific promoters driving (1) cell surface to induce differentiation of specific cell lineages; (2) cell-type-specific transcription factors to induce differentiation of specific cell lineages; (3) reporters to track cell differentiation process.